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eBook Cell-free Protein Synthesis: Methods and Protocols download

by Alexander S. Spirin,James R. Swartz

eBook Cell-free Protein Synthesis: Methods and Protocols download ISBN: 3527316493
Author: Alexander S. Spirin,James R. Swartz
Publisher: Wiley-VCH; 1 edition (December 3, 2007)
Language: English
Pages: 262
ePub: 1924 kb
Fb2: 1696 kb
Rating: 4.2
Other formats: doc docx lit lrf
Category: Medics
Subcategory: Medicine

Alexander S. Spirin, James R. Swartz. With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field

Alexander S. With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike. Categories: Biology\Biotechnology.

Cell-free protein synthesis, also known as in vitro protein synthesis or CFPS, is the production of protein using biological machinery in a cell-free system, that is, without the use of living cells. The in vitro protein synthesis environment is not. The in vitro protein synthesis environment is not constrained by a cell wall or homeostasis conditions necessary to maintain cell viability.

Cell-Free Protein Synthesis book. Start by marking Cell-Free Protein Synthesis: Methods and Protocols as Want to Read

Cell-Free Protein Synthesis book. Start by marking Cell-Free Protein Synthesis: Methods and Protocols as Want to Read: Want to Read savin. ant to Read.

Alexander S. He is one of the pioneers in the biochemical analysis of protein synthesis and has worked in the field for more than 40 years, performing the first-ever in vitro synthesis of proteins by using ribosome components. Professor Spirin has received many honors for his scientific achievements, including the Hans Adolf Krebs Medal, and is the author of several books on ribosomes and protein synthesis.

Alexander Spirin is a Professor at the Moscow State University, and the .

This protocol presents a cell-free protein synthesis method using a novel DNA gel that dramatically increases protein yield compared with current systems. This protein-producing gel (termed 'P-gel system' or 'P-gel'), which consists of genes as part of the gel scaffolding, can produce mg ml(-1) amounts of functional proteins. This protocol describes steps pertaining to plasmid design, fabrication of P-gel molds, formation of P-gel micropads and cell-free protein expression with an expected yield of up to 5 mg ml(-1) of functional Renilla luciferase (Rluc).

By: Alexander S. Spirin; James R. Publisher: Wiley-VCH. Print ISBN: 9783527316496, 3527316493.

Save up to 80% by choosing the eTextbook option for ISBN: 9783527691500, 3527691502. The print version of this textbook is ISBN: 9783527316496, 3527316493. By: Alexander S.

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With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.