carnevalemanfredonia.it
» » GTPase Protocols: The Ras Superfamily (Methods in Molecular Biology)

eBook GTPase Protocols: The Ras Superfamily (Methods in Molecular Biology) download

by Ed Manser,Thomas Leung

eBook GTPase Protocols: The Ras Superfamily (Methods in Molecular Biology) download ISBN: 089603934X
Author: Ed Manser,Thomas Leung
Publisher: Humana Press; 2002 edition (June 5, 2002)
Language: English
Pages: 267
ePub: 1803 kb
Fb2: 1624 kb
Rating: 4.5
Other formats: mbr docx lrf lrf
Category: Medics
Subcategory: Medicine

combination of clear practical detail with underlying theory provides the reader with the information required not only to perform the technique but also to interpret the resulting data. The authors are to be congratulated on the clarity brought to each techniqu. .Harry Mellor, Journal of Cell Science, Vol. 116 (7-8), 2003).

Edward J. Manser and Thomas Leung have collected the key techniques currently in use to probe the function of. Manser and Thomas Leung have collected the key techniques currently in use to probe the function of these ubiquitous proteins both in vitro and in vivo. Timely and highly practical, GTPase Protocols: The Ras Superfamily illuminates the powerful techniques used by investigators today to study this special family of proteins that plays such important roles in human health and disease.

Start by marking Methods in Molecular Biology, Volume 189: Gtpase .

Start by marking Methods in Molecular Biology, Volume 189: Gtpase Protocols: The Ras Superfamily as Want to Read: Want to Read savin. ant to Read. Edward J.

Series: Methods in Molecular biology. Other readers will always be interested in your opinion of the books you've read. Whether you've loved the book or not, if you give your honest and detailed thoughts then people will find new books that are right for them.

In this section, we describe the large-scale preparation method of. three different DNA templates used for in liposome protein syn-. thesis reaction (see Note 1). pETG5tag is a plasmid encoding the.

Michelle Webb (e., Cancer Susceptibility: Methods and Protocols, Methods in Molecular Biology, vol. 653 .

GTPase Protocols: The Ras Superfamily (Methods in Molecular Biology Vol 189). GTPase Protocols: The Ras Superfamily (Methods in Molecular Biology Vol 189). Скачать (pdf, . 0 Mb).

In the 1980s the pro- oncogene Ras itself was the focus of interest, though in the 1990s this shifted to the increasing variety of Ras-related proteins.

In the last 10 years researchers have firmly established key roles for R- related GTPases in almost every aspect of cell biology. In the 1980s the pro- oncogene Ras itself was the focus of interest, though in the 1990s this shifted to the increasing variety of Ras-related proteins. In this new decade much yet needs to be done to establish the role for all the small GTPases now uncovered by the human genome project. In particular, these GTPases need to be und- stood in the appropriate biochemical and cellular contexts. In the process of trying to uncover the role of these versatile proteins, a variety of novel te- niques and methodologies has been developed. These now enable investi- tors to move easily within a diversity of fields ranging from structural studies to real-time in vivo analysis of a GTPase. In recognition of the need for access to key background methodologies, GTPase Protocols: The Ras Superfamily is devoted to techniques that are pr- ently widely used and that will continue to be the standard for researchers worldwide. Each chapter is aimed at supplying detailed methodologies to allow reproduction in any laboratory, while also providing the general pr- ciples on which the methods are based. Some of the techniques grouped in the first section apply broadly to small GTPases, whereas others in Part II are more applicable within each GTPase subfamily.