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eBook Techniques for the Automated Optimization of High Performance Liquid Chromatography Separations download

by John C. Berridge

eBook Techniques for the Automated Optimization of High Performance Liquid Chromatography Separations download ISBN: 0471908614
Author: John C. Berridge
Publisher: John Wiley & Sons Ltd (December 18, 1985)
Language: English
Pages: 216
ePub: 1794 kb
Fb2: 1387 kb
Rating: 4.5
Other formats: lrf mbr doc docx
Category: Math Sciences
Subcategory: Chemistry

An automatic system for optimizing the conditions for the chromatographic separation of protamines has been . E. L. Styskin, L. B. Itsikson, and E. V Braude, Practical High-Performance Liquid Chromatography, Khimiya, Moscow (1986), p. 33. Google Scholar.

An automatic system for optimizing the conditions for the chromatographic separation of protamines has been developed using Nelder and Mead's modified.

Automated Optimization in high-performance liquid chromatography. The development and optimization of liquid chromatographic (. December 1986 · Analytica Chimica Acta. separations has traditionally required an ill-defined mix of experience, intuition and guesswork. Chemometric principles are, however, being more frequently applied to all. especially in the area of rational.

This book presents some of the most important modeling and prediction techniques, along with relevant applications. Only recently did they acquire a major importance in high-performance column liquid chromatography (HPLC)

This book presents some of the most important modeling and prediction techniques, along with relevant applications. Topics include linear regression, classification, resampling methods, shrinkage approaches, tree-based methods, support vector machines, clustering, and more. Only recently did they acquire a major importance in high-performance column liquid chromatography (HPLC). The advent of monolithic silica standard- and narrow-bore columns and of several families of polymer-based monolithic columns has considerably changed the HPLC field, particularly in the area of narrow-bore columns.

Published 1985 by Wiley in Chichester, New York. Automation, High performance liquid chromatography. Includes bibliographies and index. A Wiley-Interscience publication.

Characterization of chromatographic columns using the traditional van Deemter method is limited by the .

Characterization of chromatographic columns using the traditional van Deemter method is limited by the necessity of calculating extra-column variance, issue particularly relevant when modeling asymmetrical peaks eluted from monolithic columns. cle{omNR, title {RpeakChrom: Novel R package for the automated characterization and optimization of column efficiency in high-performance liquid chromatography analysis. author {Manuel David Peris-D{'i}az and Mar{'i}a Isabel Alcoriza-Balaguer and Juan Carlos Garc{'i}a-Ca{~n}averas and Francisco Santonja and Enrique Sentandreu and Agust{'i}n Lahoz}, journal {.

By this we mean automated, high-pressure liquid chromatography in columns, with a capability for the .

By this we mean automated, high-pressure liquid chromatography in columns, with a capability for the high-resolution separation of a wide range of sample types, within times of a few minutes to perhaps an hour. Very difficult separations are often more readily achieved by liquid than by gas chromatography, because of., Two chromatographic phases in LC for selective interaction with sample molecules, versus only one in GC., A greater variety of unique column packings (stationary phases) in.

In this study, the relationship between retention parameters a and c for analytical and preparative separations was developed when the same solid adsorbent and mobile phase were used. The prediction and optimization of the preparative separation of a complex sample could be achieved by direct and simple conversion of the experimentally determined data from an analytical level.

To assess package capabilities conventional packed reverse-phase and monolithic HPLC columns were .

To assess package capabilities conventional packed reverse-phase and monolithic HPLC columns were characterized. Peaks eluted from the monolithic column showed a high value of factor asymmetry due, in part, to the contribution of extra-column factors. Such deviation can be circumvented by the two alternatives approaches implemented in the R-package. Furthermore, increased values of eddy diffusion and mass transfer kinetics terms in HETP were observed for the packed column, while accuracy was below 9% in all cases.

High-performance liquid chromatography (HPLC) is now a widely applied and established analytical technique used throughout industry and research. As a tool which the analyst counts as a standard method, it is appropriate that with the increasing management of laboratory methods via microcomputers, the linking of HPLC to such systems should be considered. The author does not intend to provide a fully comprehensive review of all the possible options which are available; but rather to assess the different approaches and explore the potential for further improvements. The aim is to improve the efficiency of HPLC in producing analytical results by optimising the sensitivity, selectivity and speed of separation - a selection of procedures and techniques are drawn together and a wide range of approaches are considered. "HPLC" will enable readers wishing to purchase equipment and/or software for automated optimisation to consult this single reference source with confidence for comparison of the available approaches.