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eBook Footprinting of Nucleic Acid-Protein Complexes (Separation, Detection, and Characterization of Biological Macromolecules) download

by Arnold Revzin

eBook Footprinting of Nucleic Acid-Protein Complexes (Separation, Detection, and Characterization of Biological Macromolecules) download ISBN: 0125865007
Author: Arnold Revzin
Publisher: Academic Press (November 3, 1993)
Language: English
Pages: 193
ePub: 1153 kb
Fb2: 1554 kb
Rating: 4.8
Other formats: docx rtf doc lrf
Category: Math Sciences
Subcategory: Biological Sciences

Separation, Detection, and Characterization of Biological .

Separation, Detection, and Characterization of Biological Macromolecules. Footprinting of Nucleic Acid-Protein Complexes. This book is a laboratory manual of footprinting techniques for studying nucleic acid-protein interactions. For the End User) Provides clear exposition of footprinting techniques for characterizing DNA-protein interactions Covers both protection methods for identifying sites of protein binding and interference methods for determining points of contact between DNA and protein Includes approaches for both in vitro and in vivo measurements High quality, timely, and of lasting practical value in the laboratory.

This book is a laboratory manual of footprinting techniques for studying nucleic acid-protein interactions. It contains clear and concise descriptions of the most important methodologies, and includes in vivo as well as in vitro applications. Use of different footprinting approaches can provide unique insights into DNA-protein systems

Proteins and Nucleic Acids. biological role of proteins, the preliminary knowledge of chemical proprieties of. amino acids is required.

Proteins and Nucleic Acids. General properties of the amino acids in relation with the structure. Amino acids are small molecules, with mean molecular weights around 100, containing an amino and a carboxyl group, bonded to an organic group, R, in those of.

Footprinting of Nucleic Acid-Protein Complexes (Separation, Detection, and Characterization of Biological Macromolecules). Footprinting of nucleic acid-protein complexes by arnold revzin. sSrlUernHddnq TBsbjuyt uZAtcxeIe oUMErpYXLQyheSl. You dismissed this ad.

This laboratory manual of footprinting techniques for studying nucleic acid-protein interactions contains . Footprinting of Nucleic Acid-Protein Complexes (Separation, Detection, and Characterization of Biological Macromolecules, Vol 1). ISBN.

This laboratory manual of footprinting techniques for studying nucleic acid-protein interactions contains descriptions of the most important methodologies and includes in vivo as well as in vitro applications. The protocols are written to be user-friendly. 0125865007 (ISBN13: 9780125865005).

Table of Contents This book is a laboratory manual of footprinting techniques for studying nucleic.

The methods are central to studying key biological processes, such as transcription and translation. The techniques are important to experiments in vivo<$ and in vitro<$, in eukaryotes and in prokaryotes, at qualitative and quantitative levels,and across many disciplines.

Macromolecules - proteins, nucleic acids, and polysaccharides - are .

Macromolecules - proteins, nucleic acids, and polysaccharides - are formed by the polymerization of hundreds of their precursors - amino acids, nucleotides, and simple sugars. The diversity among macromolecules evolves from the vast potential to form different combinations of the 50 or so common monomers which make up a macromolecule. The polysaccharides, or complex carbohydrates, represent the form that sugar takes when it is stored. Polysaccharides are the structural components of a cell.

Revzin (e. : Footprinting Techniques for Studying Nucleic Acid-Protein Complexes (A Volume of Separation, Detection, and Characterization of Biological Macromolecules) (Academic Press, New York 1993)Google Scholar.

Protein–nucleic acid interactions play key roles in many biological .

Protein–nucleic acid interactions play key roles in many biological processes. Some protein–nucleic acid complexes may persist throughout purification, making it difficult if not impossible to determine the types of nucleic acids to which the protein will bind. The denaturation step separates the two components, thereby improving association of the labeled nucleic acid. Detection and characterization of nucleic acid–protein interactions, particularly those involving DNA and proteins such as transcription factors, enzymes, and DNA packaging proteins, remain significant barriers to our understanding of genetic regulation.

This is an old book now, and I don't know if its been supplanted, but when published it was by far the best book in its field. I think it still has much to offer.

Footprinting of Nucleic Acid-Protein Complexes provides protocols for studying the stoichiometry, binding site size and location, and structural changes in nucleic acids caused by their interaction with proteins. The methods are central to studying key biological processes, such as transcription and translation. The techniques are important to experiments in vivoand in vitro, in eukaryotes and in prokaryotes, at qualitative and quantitative levels,and across many disciplines.This book is a laboratory manual of footprinting techniques for studying nucleic acid-protein interactions. It contains clear and concise descriptions of the most important methodologies, and includes in vivo as well as in vitro applications. It is aimed at bench scientists from graduate students on, and should be of value in industrial labs as well as in academic settings. Use of different footprinting approaches can provide unique insights into DNA-protein systems. The protocols containedin this handbook are written to be"user-friendly,"and thus should be conducive to extending the use of footprinting to new systems. The section on quantitative analysis of DNAse I footprints should prove especially useful for in depth evaluation of cooperative interactions. (For the End User)Provides clear exposition of footprinting techniques for characterizing DNA-protein interactionsCovers both protection methods for identifying sites of protein binding and interference methods for determining points of contact between DNA and proteinIncludes approaches for both in vitro and in vivo measurementsHigh quality, timely, and of lasting practical value in the laboratory