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eBook RNA Abundance Analysis: Methods and Protocols (Methods in Molecular Biology, Vol. 883) download

by Hailing Jin,Walter Gassmann

eBook RNA Abundance Analysis: Methods and Protocols (Methods in Molecular Biology, Vol. 883) download ISBN: 161779838X
Author: Hailing Jin,Walter Gassmann
Publisher: Humana Press; 1st edition (May 21, 2012)
Language: English
Pages: 231
ePub: 1610 kb
Fb2: 1423 kb
Rating: 4.5
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Category: Different
Subcategory: Medicine and Health Sciences

Methods and Protocols

Methods and Protocols. RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Part of the Methods in Molecular Biology book series (MIMB, volume 883). In: Jin . Gassmann W. (eds) RNA Abundance Analysis. Methods in Molecular Biology (Methods and Protocols), vol 883. Humana Press, Totowa, NJ. First Online 06 April 2012. Often the depth and breadth of coverage of RNA-seq data can exceed what is achievable using microarrays. However, the strengths of RNA-seq are often its greatest weaknesses.

The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known.

The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Nutrients for RNA replication and membranes for liposome proliferation were combined by using a modified freeze-thaw technique.

In molecular biology, b-glucuronidase has been used as a reporter gene to monitor gene. expression, because it transforms certain substrates into colored. Nutrients for RNA replication and membranes for liposome proliferation were combined by using a modified freeze-thaw technique. These liposomes showed fusion and fission compatible with RNA replication and distribution to daughter liposomes.

Metagenomics: Methods and Protocols - Methods in Molecular Biology Vol 668. Wolfgang R. Streit. Download (pdf, 2. 0 Mb) Donate Read.

Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Series: Methods in Molecular Biology (Book 902). Hardcover: 224 pages.

Wide ranging and highly practical, Stress Response: Methods and Protocols provides a gold-standard bench manual for today's basic and clinical scientists working to understand how cells and tissues respond during physiological stress and in human disease. One of the challenges is to enhance the phospholipids (PL) content, and another is to enhance the content of specific water soluble nutrients, like free amino acids (FAA). There are a few studies where this has been achieved by the use of liposomes.

'Patch-clamp: Methods and Protocols’ introduces some common patch-clamp applications. It is intended to assist scientists in identifying problems, which could be best addressed by this technique. The book provides the step-by-step procedures to perform the experiments that answer those questions. It will be a useful companion for moderately advanced users wishing to extend their skills. Maria Drigelova, General Physiology and Biophysics, Vol. 27, April, 2008). Patch Clamp Methods and Protocols surveys the typical patch clamp applications and.

Ali Masoudi- Nejad, Zahra Narimani, Nazanin Hosseinkhan (2013) Next Generation Sequencing and Sequence Assembly: Methodologies and Algorithms (Springer Briefs in Systems Biology) ISBN- 10: Eberhard O. Voit (2012) A First Course in Systems Biology. ISBN It is also a very good introduction to biology for computer scientists

Methods in Molecular Biology™ is a trademark of The Humana Press Inc. The content and opinions expressed in this book are the sole work of the .

p. cm. - (Methods in molecular biology ; 212) Includes bibliographical references and index.

RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. In RNA Abundance Analysis: Methods and Protocols, expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches.  This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets.  Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Essential and easy to use, RNA Abundance Analysis: Methods and Protocols provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and modified RNAs, which can assist you in your gene expression studies.