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eBook In Vitro Transcription and Translation Protocols (Methods in Molecular Biology) download

by Guido Grandi

eBook In Vitro Transcription and Translation Protocols (Methods in Molecular Biology) download ISBN: 1588295583
Author: Guido Grandi
Publisher: Humana Press; 2nd edition (May 3, 2007)
Language: English
Pages: 276
ePub: 1341 kb
Fb2: 1196 kb
Rating: 4.6
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Category: Different
Subcategory: Medicine and Health Sciences

Methods in Molecular Biology.

Methods in Molecular Biology. In Vitro Transcription and Translation Protocols.

Chapter · January 2003 with 36 Reads. Cite this publication. Farahnaz Movahedzadeh. Free delivery worldwide. Completely updated with new chapters and topics, there is in-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems.

In vitro transcription requires a purified linear DNA template containing a promoter, ribonucleotide triphosphates, a buffer system that includes DTT and magnesium ions, and an appropriate phage RNA polymerase. The exact conditions used in the transcription reaction depend on the amount of RNA needed for a specific application. Top. Translation systems. Rabbit reticulocyte lysate Rabbit reticulocyte lysate is a highly efficient in vitro eukaryotic protein synthesis system used for translation of exogenous RNAs (either natural or generated in vitro).

Life Sciences - Biology - Molecular Biology, Genetic transcription, Genetic translation, Laboratory manuals, Molecular genetics.

Genetics (non-medical), Science, Science/Mathematics, Life Sciences - Biochemistry, Life Sciences - Cytology, Life Sciences - Genetics & Genomics, Science, Genetics, Life Sciences - Biology - Molecular Biology, Genetic transcription, Genetic translation, Laboratory manuals, Molecular genetics.

Автор: Guido Grandi Название: In Vitro Transcription and Translation Protocols ISBN: 1617376418 . It is devoted to prokaryotic transcription and is useful for use on molecular biology, microbiology and technology courses

It is devoted to prokaryotic transcription and is useful for use on molecular biology, microbiology and technology courses.

In most mammals, BAT develops during gestation and perinatal life (26). It is prominent in the newborn or in young animals in which nonshivering ther-mogenesis is necessary to counteract heat loss associated with birth and atmo-spheric life.

A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. Completely updated with new chapters and topics, there is in-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems. Detailed protocols for protein production using different in vitro transcription and translation systems are included.